For verification of this paper, please visit on http://www.socialresearchfoundation.com/innovation.php#8

The green plants synthesize food in the form of glucose through photosynthesis. Chlorophyll is considered as main pigment molecule that facilitates the synthesis of food for plants with the help of sunlight and many other factors. Proteins are building blocks of the body of the organisms and are involved in normal growth and development of plants. Legumes and pulses are good source of proteins for humans. The excessive use of pesticides in the fields adversely affects chlorophyll and protein contents of crop plants. Quantitative analysis of chlorophyll and protein in pulse plants gives an idea of photosynthesis capacities and nitrogen content of the plants. Therefore, for the present study, the effect of increasing concentration of rogor on Vigna mungo is analyzed to estimate the content of chlorophyll and proteins. For chlorophyll and protein estimation, the seedlings were harvested on third sampling stage. The plant tissues were weighed and analyzed for these parameters on mg/g fresh weight basis. Chlorophyll content was estimated by extracting in 80% acetone and determined spectrophotometrically following the procedure of Arnon (1949). The protein content was also estimated spectrophotometrically by the method of Bradford (1976). On the basis of present studies, it is concluded that in Vigna mungo, chlorophyll a, chlorophyll b, total chlorophyll and total protein contents decreased with increasing concentration of pesticides, Thus, higher concentrations of pesticides are detrimental to these legumes.

The present study, the effect of increasing concentration of rogor on Vigna mungo is analyzed to estimate the content of chlorophyll and proteins in the selected plant.

In the year 1993 Kannabrian B and Pragasam A studied the effect of distillery effluent on seed germination, seedling growth and pigment content of Vigna mungo. Kannabrian, B. and Pragasam, A. (1993) worked on effect of distillery effluent on seed germination, seedling growth and pigment content of Vigna mungo (L.), In 1987 Neeta Srivastav and Sahai. Studied the effects of distillery waste on the performance of Cicer arietinum L. In the year 2002 Mishra Virendra, Pandey Shridhar gave the effect of distillery effluent on leachates of industrial sludge on the germination of black gram (Cicer arrietinum) Pandey S D worked on the effect of distillery effluent and of industrial sludge on the germination of black gram germination, seedling growth and pigment content of Vigna mungo (L.), In 1987 Neeta Srivastav and Sahai. Studied the effects of distillery waste on the performance of Cicer arietinum L.

For chlorophyll and protein estimation, the seedlings were harvested on third sampling stage. The plant tissues were weighed and analyzed for these parameters on mg/g fresh weight basis. Chlorophyll content was estimated by extracting in 80% acetone and determined spectrophotometrically following the procedure of Arnon (1949). The protein content was also estimated spectrophotometrically by the method of Bradford (1976).

The results of the effect of rogor on Vigna mungo, sample I are shown in table 1 and figure 1A, B, C, and D. The data show that the chlorophyll ‘a’ content was 0.987, 0.970, 0.896 mg/g, and 0.813, 0.742, 0.660 mg/g, and 0.542, 0.321 mg/gm fresh weight of tissue in control and respective concentrations of pesticide. The chlorophyll ’b’ contents were 0.548, 0.516, 0.466 mg/g, and 0.377, 0.312, 0.220 mg/g, and 0.512 and 0.103 mg/gm fresh weight of tissue in control and respective concentrations of pesticide.  The total   chlorophyll contents were 0.664, 1.650, 1.490 mg/g, and 1.350, 1.210, 1.080 mg/g, and 0.880, 0.510 mg/gm of fresh tissue and the total protein contents were 28.45, 28.42, 24.46 mg/g, and 22.95, 19.75, 14.60 mg/g, and 11.47 and 9.54 mg/gm fresh weight of tissue for increasing concentrations of rogor, i.e., 0.0%, 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 5.0%, 7.5% respectively. No results were obtained for 10.0% concentration.

Table 2 and fig. 1 A, B, C, and D show the effect of rogor on Vigna mungo, sample II. The data show that the chlorophyll ‘a ‘contents were 0.960, 0.950, 0.891 mg/g, and 0.780, 0.720, 0.640 mg/g, and 0.520, 0.319 mg/g while chlorophyll ‘b’ contents were 0.510, 0.500, 0.464 mg/g, and 0.360, 0.290, 0.180 mg/g, and 0.130, 0.104 mg/gm fresh weight of tissue in control and respective increasing concentrations of pesticide. The total chlorophyll contents were 1.64, 1.63, 1.47, 1.34 mg/g, and 1.20, 1.14, 0.89 mg/g, and 0.51 mg/g and the total protein contents were 27.90, 26.80, 24.47 mg/g, and 23.10, 19.50, 14.70 mg/g, and 11.40, 9.56 mg/gm fresh weight of tissue for increasing concentrations of rogor, i.e., 0.0%, 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 5.0% and 7.5% respectively. No results were obtained for 10.0% rogor concentration.

 

On the basis of present studies, it is concluded that in Vigna mungo, chlorophyll ‘a’, chlorophyll ‘b’, total chlorophyll and total protein contents decreased with increasing concentration of pesticides, i.e., 0.0%, 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 5.0% and 7.5% and at 10.0% concentration, no results were obtained. Thus, higher concentrations of pesticides are detrimental to these legumes.

1. Kannabrian B and Pragasam A (1993) Effect of distillery effluent on seed germination, seedling growth and pigment content of Vigna mungo (L.), Hepper, Geobios, 20: 108-112. 2. Kannabrian, B. and Pragasam, A. (1993), Effect of distillery effluent on seed germination, seedling growth and pigment content of Vigna mungo (L.), Hepper. Geobios, 20: 108-112. 3. Neeta Srivastav and Sahai. (1987) Effects of distillery waste on the performance of Cicer arietinum L. Environmental pollution vol 43, (2) 99-102. 4. Mishra V and Pandey S D (2002) Effect of distillery effluent and of industrial sludge on the germination of black gram (Cicer arietinum). Pollu. Res. 21(4): 461-467. 5. Mishra Virendra, Pandey Shridhar. Effect of distillery effluent on leachates of industrial sludge on the germination of black gram (Cicer arrietinum) Polln Res, 21(4): 461-467.