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In Dysdercus koenigii six haemocytes are reported in these one is plasmatocytes(PLs). The haemocytes are cellular elements of the haemolymph those are associated to number of physiological and biochemical activities of the insects. In the present work it is observed that the fluctuations in plasmatocytes(PLs) are directly associated to injuries effect and the healing process of injuries. Initially up to day 2 slightly more plasmatocytes (PLs) reported in experimental insect comparison to the controls. But after day 2 to up to day 6 significantly higher plasmatoytes(PLs) reported in the experimental insects than to the controls. After day 6 plasmatocytes(PLs) numbers in more or less same in both the experimental and control insects.

To study the roles of plasmatocytes in defense of the insects body particularly in relation to injury and heeling of injury processes. The present study also carried in objective these heamocytes may use as experimental materials for the immune related study of human being.

For the present study number of literatures and findings are reviewed and as conclusion it is noted very little work has been carried out on the topic the role of plasmatocytes in relation to injury of Dysdercus koenigii. The reports of Mishra and Tiwari 2005, Pandey and Tiwari 2012, Rizki 1957, Merchant et al 2008, Liegeois and Ferrandon 2020, Kurcuz et al 2003, Gupta 1979, Greeny 2012 and Berger et al 2003 papers are reviewed.

For the present work the red cotton bug Dysdercus koenigii are collected from the fields nearby of cotton plants and lady finger’s plants from villages surrounding of our college. These insects are cultured in BOD at 28±1°C, 75% RH and 16hr of photoperiod. In cultured jars insects were feed on soaked cotton seed and get water from small vials. For the present work “0”hr of freshly molted females were sorted out and they divided into two groups one experimental and other as controls. In the experimental group a 1 mm fine injury made on neck of the insect by sharp scalpel blade and by forceps some fat body removed from the incision. In controls only touch the neck region and physical stress given by scalpel blade not made incision. After 24 hr of interval 10 insects were collected by cutting antenna a drop of haemolymph taken on slide made a smear, stained them by Giemsa and counted 100 haemocytes and note the plasmatocytes numbers in these 100 cells and marked them as %.

From the study of Table 1 it is shown that from day 2 to up to day 6 plasmatocytes numbers are significantly higher in the experimental insect comparison to the controls. After day 6 numbers of plasamatocytes still higher in the experimental insects but it not significant. In the initial days the haemocytes; particularly plasmatocytes (PLs) are more or less same in both the group of insects.

Table: 1 (Fluctuations in Plasmatocytes (PLs) numbers of adult female Dysdercus koenigii )

S.N	Age in days	Experimental (% of PLs)	Controls (% of PLs)	P Value
1	1	36.8 ±3.82	34.7±3.72	N S
2	2	38.4± 3.86	35.9± 3.91	N S
3	3	39.1±3.88	33.1± 3.89	<0.01
4	4	41.2±4.01	32.8 ±2.98	“
5	5	42.9± 4.12	31.6 ±3.01	“
6	6	44.1±4.23	29.9±2.47	“
7	7	41.6 ±4.19	34.7 ±3.12	“
8	8	36.7 ±3.87	35.8 ±3.13	N S

In the present study the higher plasmatocytes (PLs) from day 0 to day 8 in the experimental insects are reveals the role of PLs in the injury and injury associated activities of Dysdercus koenigii. The report of Pandey and Tiwari 2015 stated the higher PLs are associated to egg maturation in adult female. But in the present case it not be true because PLs are significantly higher to controls further the reports of Gupta 1979 and Rizki 1957 they stated the inter conversion of other types of haemocytes increase the PLs. This assumption not clearly reveals the fact in the present study. The present findings support the reports of Liegeeis and Ferrandon 2020 that stated PLs and its sub types involve in immunity. 

Plasmatocytes (PLs) plays roles in defense activities of Dsdercus koenigii. Higher PLs are noted in the experimental insects than to the controls.

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