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Inorganic chemistry applied to Biomedical sciences has emerged as a recent off shoot of bioinorganic/medicinal – inorganic chemistry Schiff bases offer opportunities for including substrate chirality, tuning the metal centered electronic factor, enhancing solubility and either performing homogeneous or hetero-geneous catalyzes and diversified subjects including bi- coordination and bio- inorganic chemistry. In Co(II) complex with the new Schiff bases of the type P –HBAT (C10H8N2OS) and P-HBAMP (C13H12N2O) were prepared by reaction of P-hydroxy benzaldehyde with 2amino thiazole/ 2-amino-6-methyl pyridine have been synthesized and characterized by elemental analysis, IR and electronic spectral data. Ligand and complexes have been screened for their anti bacterial activities.

The present work report the synthesis and characterization of Co(II) complexes with new Schiff bases have been synthesized. The structures were confirmed by IR, mass, NMR, elemental, and electronic spectral data. Ligand and complexes have been screened for their activities.

Aromatic ring structures attached to each other through an element or a group such as C-C , O-S and CH2­ increase the activity of the resultant compound several fold , It has been reported that 5 or 6 member ring with heteroatom such as O,N and S impart Special biological activity of the compound 8 – 9. The metal complexes play an important role in biological system in which enzyme are known to be activated by the metal ions.

Experimental
Synthesis of Schiff bases
(a) Schiff base derived from p-Hydroxy benzalidene with 2 – amino thiazole (SB₁) 

(p-Hydroxy benzalidene p -HBAT2 – amino thiazole)(SB₁)(b) Schiff base derived from p-Hydroxy benzalidene with -2– amino-6- methyl pyridine (SB₂)

(p-Hydroxy benzalidene -2–  p -HBAMP amino-6- methyl pyridine)(SB₂)
Synthesis of metal complexes :
(a) Preparation of metal complexes with p-HBAT (SB₁)

(b) Preparation of metal complexes with p-HBAMP (SB₂)

Where M = Co(II) 
X = Cl^-
Physical Measurements 
1. Infrared spectra – The infrared spectra of the ligand  and their metal complexes were recorded at perkin Elmer spectrophotometer in the region 4000-200 Cm^-1 from St. Johns’s College, Agra.
2. Mass spectra – The mass spectra of the ligands were recorded in the region 100 – 350 MHz at the C.S.I.F., CD.RI Lucknow.
3. N.M.R. spectra – The N.M.R. spectra of all the ligands were recorded at C.S.I.F, CDR.I. Lucknow.
4. Electronic spectra – The electronic spectra of all the complexes were recorded at Eleco. SL- 171 – spectrophotometer at the room temperature in the range 320 – 1000 nm in the department of chemistry, I.B.S. Khandari, Agra.
Analytical Technique 
1. Micro Analysis of Carbon, Hydrogen and Nitrogen
For the micro analysis of carbon, hydrogen and nitrogen C,H,N, micro analyzer were used the analyses were done at C.S.I.F., CD.R.I., Lucknow.
2. I.R. Spectra
Infrared spectroscopy play an important  role in the study of polydentate ligands containing N, O and S as donor atoms greater attention on has been paid on the study of the metal ligand vibrations foar infrared region.[8]

Table-1
Important infrared  frequencies cm^-1 schiff base p- hydroxybenzaldehyde -2-amino thiazde and its metal complexes.

Table -2
Important infrared  frequencies cm^-1 schiff base p- hydroxybenzaldehyde -2-amino – 6- methylpyridene and its metal complexes.

MASS Spectra
Mass spectrometry is applicable to a wide variety of problems in inorganic and physical chemistry. A large number of worker have reported mass spectral studies of Schiff based^{9 – 11} and their metal complexes.

(A) The possible modes of mass fragmentation of Schiff base p-hydroxy benzaldehyde-2-aminothiazole (SB₁) :

(B) The possible modes of mass fragmentation of Schiff base p-hydroxy benzaldehyde-2-amino – 6- methyl pyridine (SB₂) :

N.M.R. SPECTRA 
Nuclear magnetic resonance is a branch of spectroscopy in which radio frequency waves induce transitions between magnetic energy levels of nuclei of a molecule[12-14]. 

Table-1
NMR spectral data of Schiff bases p-hydroxy benzaldehyde -2 – aminothiazole

Table-2
NMR spectral data of Schiff bases p-hydroxy benzaldehyde-2-amino-6- methyl pyridine :

Spectral data and computed values of spectral parameters for Co(II) complexes.

Anti Bacterial Activity
The  increase in the morlality  rate associated with infectious disease is directly related to bacteria that exhibit multiple resistance to antibiotics. The lack of effective treatments is the main cause of this problems.^{(15-16) .} Over the recent few years, from 2015 to present the Schiff-base metal complexes have earned much attention due to the biological properties of them. A large number of studies have been publisheshed on their use in biological applications.¹⁷The percentage inhibition of the Schiff bases and metal complexes are recorded in the table 1 & 2. All the complexes are active against the bacteria species.
Table-1
Anti Bacterial Activity Data Against E.Cole
(inhibition of radial growth in mm)

Table-2
Anti Bacterial Activity Data Against S. Aureus
(inhibition of radial growth in mm)

The activity of newly synthesized Schiff base ligand is also lesser than that of its complexes. The comparative antimicrobial evaluation reveals that the Schiff base. C10H8N2OS is almost inactive against E.Coli at the concentration of 250ppm and 500ppm, while it showed optimum activity at 750ppm. The activity of Co(II) complexes increase with conc. i.e. at 250ppm they are less active but at 750ppm they showed good activity. The perusal of Table-2 shows that Co(II) complexes exhibit enhanced activity than Schiff base ligand C13H12N2O , against S aureus at 250ppm all metal complexes showed lesser activity and at 750ppm they showed higher activity.

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