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Antifungal Effect of
Leaf Extracts Against F. oxysporum in Ridge Gourd Seeds |
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Paper Id :
18074 Submission Date :
2022-07-13 Acceptance Date :
2022-07-19 Publication Date :
2022-07-25
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Abstract |
Leaf extracts of 11 plant species were used for
controlling infection of F. oxysporum in ridge gourd seeds. All the
plant extracts enhanced seed germination and inhibited growth of pathogen on
seed and seed-ling surface and proved superior for controlling incidence of the
pathogen. |
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Keywords | Chemical Fungicides, Pathogen Effect, Leaf Extracts, F.oxysporum. | ||||||
Introduction | F. oxysporum is a very common pathogen causing wilt disease and affect the crop yield. Bhatia et.al. (1995), Dubey & Singh (2005) have studied inhibitory effect of pathogen in germination of seed of crops like guar and sesame respectively. More than 2000 species belonging to77 families are known to possess antifungal properties. Singh et al. (1994), Navarro Garco et al. (2003), Sharma & Bohra (2003), Varma & Kharwar (2006) have studied various plant species for their antifungal activity. Use of chemical fungicides to control the pathogen effect create some
disadvantages such as high toxicity to targeted organisms, environmental
pollution and residual hazards in soil. |
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Objective of study |
Keeping in view of above problems, the present study was undertaken in which effects of eco-friendly methods to control the severe pathogen developed in seeds by using different leaf extracts. |
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Review of Literature | Review of literature has given in heading result and
discussion . |
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Methodology | Fresh leaves of 11 plants viz. Azadirachta indica,
Calotropis procera, Cassia tora, Catharanthus roseus, Dalbergia sissoo, Datura
innoxia, Eucalyptus rudis, Lantana camera, Lawsonia inermis, Parthinium
officinalis, Riccinus communis were tried for treatment. 5 gm fresh leaves
of each plant were crushed separately in 20 ml distilled water using pastle and
mortor. The extracts were filtered through double layer of cheese cloth. Five
replicates of 10 seed (surface sterilized with 2% sodium hypochlorite were
taken randomly and treated with each of 11 aqueous extracts for four h. Ten
seeds per plates were sown on sterilized moistened blotter for 8 days in
Petriplate. The plates were incubated under 12 h alternating cycles of light
and dark for 8 days (Anonymous,1985). Observations were made under Nikon Stereo
binocular microscope. The data on seed germination, incidence of pathogen and
infected seedlings were recorded after every 24 h intervals up to 8 days. The
seed-ling without any symptom showing normal seed-ling growth and vigour were
considered as uninfected. Seeds soaked in distilled water served as control.
The percentage of seed germination, seed-ling infection and control of pathogen
or percent inhibition of pathogen incidence was calculated. |
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Result and Discussion |
The germination was effectively enhanced by all the
plant extracts used. Dalbergia sissoo showed maximum effect 94% against F.
oxysporum followed by Lawsonia 86%Cassia 84% Catharanthus,Datura
and Parthinium 80%, Azadirachta, Calotropis and Lantana76%. Best infection control of F. oxysporum was
achieved by Dalbergia sissoo (90.90%) followed by Azadirachta indica, Calotropis procera, Lawsonia inermis (86.36%), Eucalyptus
and Lantana camera (81.81%). In other plant extract it ranged from
(72.72-77.77%). Incidence of pathogen was significantly controlled by all the 11 leaf
extract and was maximum (91.66%) in Dalbergia sissoo and Cassia tora
(Fig.A&B). Similarly Gupta et al. (1996) evaluated efficacy of leaf
extracts of Azadirachta indica, Calotropis procera, Eucalyptus rudis,
Parthinium hysterophorus and Pongemia
pinnetta against Fusarium oxysporum
sp. from diseased malberry plant . Mantha spicata
has also been found to have antifungal properties against F. oxyporum by
Singh et al. (1994). Sharma & Triwedi (2002) have also recommended
many leaf extracts to control nematodal disease of crop plants caused by Meloidogyne
incognita and also fungi F.oxysporum in cumin plant.
Ogachi et al. (2006) has also studied antifungal effect of some plant extracts against F. oxysporum to control tomato wilt and found neem extract was the most effective (100%) to inhibit the mycelial growth of F. oxysporum. Tinospora cordifolia, Cymbopogon citratus and Moringa olifera showed promising antifungal potentiality against F. oxysporum sp. Ciceri with maximum inhibition in chick pea. (Dwivedi and Sangeeta,2015). |
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Conclusion |
Plant extracts are natural, herbal antifungal
materials. Because of their easy availability, low cost and easy
biodegradability these can be easily used to control seed-borne pathogen. In
the present study all leaf extracts gave good results to control seed-borne
pathogen. |
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References | 1. Anonymous 1985 International Rules of Seed Testing.
International Seed Testing Association. Seed Science and Tecnol.13;299-513 2. Bhatia A, Singh, T & Singh D 1995 Infection of F.oxysporum
in guar (Cyamopsis tetragonoloba (L) Taub. ) seeds. Acta Botanical
India 23;257-259 3. Dubey A and Singh T 2005. Incidence, location and
transmission of seed-borne inoculum of Fusarium moniliforme in sesame.
J. Mycol.Pl. Pathol. 35(1)12-15. 4. Dwivedi, S.K. and Sangeeta2015.Efficacy of some
medicinal plant extract against F. oxysporum f. cicero causing Chick pea
wilt .7(2)138-146. 5. Gupta V.; Govindaiah & Datta, R.K.1996 Plant extract
a nonchemical approach to control Fusarium disease of Malberry. Current
Science 71(5);406-40 6. Navarro Garco, V.M.; Gonzalez, A.; Fruntes, M.; Aviles,
M.; Rios, M.M.; Zepada, G.; Rajas, M.G. 2003. Antifungal activities of nine
traditional Maxican plants. J. Ethanipharmacol.87(1);85-87. (4) 7. Ogachi,N; Agbenin; P.S. Marley 2006. In vitro assay
of some plant extract against F. oxysporum f. sp. Lycopersici causal
agent of tomato wilt Journal of Plant Protection. 46(3)215-220 8. Sharma,S and Bohra ,A.2003. Effect of extracts of
some medicinal plants on F. oxysporum var. cumini. J. Mycol and Plant
Pathol.33(2);323-324. 9. Sharma N & Triwedi PC 2002 Screening of leaf
extracts of some plants for their nematicidal and fungicidal properties against
Maloidogye incognita and F. oxysporum. Asian J.Exp.Sci.
16(1&2);21-28. 10. Singh, J; Dubey, A.K Tripathi, N.N.1994 antifungal
activity of Mentha spicata. Int.J. Pharmocog.32(4);314-319.
11. Varma, C vijay and Kharwar N Ravindra
2006.Efficacy of neem leaf extract against its own fungal endophytic Curvularia
lunata. Journal of Agriculture Technology 2(2);329-335. |